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KMID : 0881720190340050495
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Journal of Food Hygiene and Safety
2019 Volume.34 No. 5 p.495 ~ p.501
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Induction of Apoptosis in HT-29 Human Colorectal Cancer by Aloin
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Yoo Eun-Seon
Woo Joong-Seok
Kim Sung-Hyun
Lee Jae-Han
Han So-Hee
Jung Soo-Hyun
Park Young-Suk
Kim Byeong-Soo
Kim Sang-Ki
Park Byung-Kwon
Jung Ji-Youn
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Abstract
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Aloin [1,8-Dihydroxy-10-(β-D-glucopyranosyl)-3-(hydroxymethyl)-9(10H)-anthracenone], is a natural anthraquinone from aloe. It has been shown to have antioxidant and anticancer effects in various types of human cancer cells, but the anticancer effects of aloin in human colorectal cancer cells HT-29 have not been elucidated. In this study, possible mechanisms by which aloin exerts its apoptotic action in cultured human colorectal cancer HT-29 cells were investigated. The results of 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay shows that treatment with aloin (0, 100, 200, 300 and 400 μM) reduced cell viability in a concentrationdependent manner in HT-29 and showed no effects on cell proliferation in A375SM and AGS cells. In addition, it was confirmed that apoptotic body was significantly increased as shown by 4’,6-diamidino-2-phenylindole (DAPI) staining, and increased apoptosis rate by flow cytometry in HT-29 cells treated with aloin (0, 200 and 400 μM). We confirmed by western blotting that aloin activated Bax (pro-apoptotic), cleaved-poly (ADP-ribose) polymerase (PARP) and caspase-3, -8 and Bcl-2 (anti-apoptotic) were not changed compared with the control. Aloin induced up-regulation of phospho-p38 and down-regulation of phospho-extracellular signal-regulated kinase (ERK)1/2. Therefore, aloin suppressed the growth inhibitory effects by the induction of apoptosis in human colorectal cancer cells and has potential as a cancer preventive medicine.
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KEYWORD
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Aloin, Apoptosis, HT-29, p38 MAPK pathway, Anticancer
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